3CHO
Crystal structure of leukotriene a4 hydrolase in complex with 2-amino-N-[4-(phenylmethoxy)phenyl]-acetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.076 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.312, 87.124, 99.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.740 - 1.800 |
| Rwork | 0.188 |
| R-free | 0.22300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.740 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 62985 | |
| <I/σ(I)> | 10.4 | 2.9 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 298 | PEG 8000, SODIUM ACETATE, IMIDEAZOLE PH 6.8, YBCL2, BESTATIN, LIQUID DIFFUSION, TEMPERATURE 298K, pH 8.00 |
