3CHM

Crystal structure of PCI domain from A. thaliana COP9 signalosome subunit 7 (CSN7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	110
Detector technology	CCD
Collection date	2006-11-30
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	C 2 2 21
Unit cell lengths	57.094, 85.796, 72.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.900 - 1.500
R-factor	0.16559
Rwork	0.164
R-free	0.19996
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	28844
<I/σ(I)>	47.9	6.7
Completeness [%]	99.5	97.1
Redundancy	8.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	292	23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	7.2	292	23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	7.2	292	23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K