3CHL
Crystal Structure of Alpha-14 Giardin with magnesium bound
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Detector technology | CCD |
Collection date | 2007-05-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979, 0.91216, 0.9793, 0.9795 |
Spacegroup name | H 3 |
Unit cell lengths | 133.859, 133.859, 69.668 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.465 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE (2.09) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 66.960 |
High resolution limit [Å] | 1.900 |
Number of reflections | 36608 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 273 | 100 mM magnesium chloride, 100 mM sodium citrate pH 5.0, and 15% (w/v) PEG 4000 , VAPOR DIFFUSION, SITTING DROP, temperature 273K |