3CGY

Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-27
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	87.923, 63.759, 87.772
Unit cell angles	90.00, 121.41, 90.00

Refinement procedure

Resolution	30.000 - 2.600
Rwork	0.246
R-free	0.29900
Starting model (for MR)	1id0
RMSD bond length	0.010
RMSD bond angle	1.644
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.073	0.465
Number of reflections	12655
<I/σ(I)>	9.4	2.2
Completeness [%]	98.1	97.9
Redundancy	3.6	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	27.5% PEG 4000, 0.1M MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K