3CGR

X-ray structure containing the pseudouridylated U2 snRNA and intron branch site consensus sequences

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-10
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	67.980, 40.470, 32.210
Unit cell angles	90.00, 95.20, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.2415
Rwork	0.238
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	our previously solved RNA duplex
RMSD bond length	0.005
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	4759
<I/σ(I)>	12	2.5
Completeness [%]	93.1	83.2
Redundancy	5.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulfate
2	1	2	ammonium sulfate