3CGL
Crystal Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.202, 78.269, 188.566 |
Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.090 |
Rwork | 0.185 |
R-free | 0.21668 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1G7K |
RMSD bond length | 0.011 |
RMSD bond angle | 1.520 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.090 | 2.100 |
Rmerge | 0.070 | |
Number of reflections | 90778 | |
<I/σ(I)> | 21.8 | 5.4 |
Completeness [%] | 94.8 | 67.5 |
Redundancy | 7 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 100 mM HEPES pH 7.9, 300 mM NaCl, 200 mM calcium acetate, 16% v/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |