3CGG

Crystal structure of TehB-like SAM-dependent methyltransferase (NP_600671.1) from Corynebacterium glutamicum ATCC 13032 Kitasato at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97978
Spacegroup name	P 21 21 21
Unit cell lengths	51.330, 87.398, 92.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.062 - 2.000
R-factor	0.169
Rwork	0.167
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.827
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.062	29.062	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.093	0.048	0.667
Total number of observations		1136	7762
Number of reflections	28773
<I/σ(I)>	6.6	11.7	1.1
Completeness [%]	99.9	94.7	100
Redundancy	3.6	3.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	NANODROP, 1.0M Sodium citrate, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K