3CG7
Crystal structure of cell-death related nuclease 4 (CRN-4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.999 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.474, 165.379, 64.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.20816 |
Rwork | 0.206 |
R-free | 0.24681 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.426 | |
Number of reflections | 45644 | |
<I/σ(I)> | 21.5 | 3.01 |
Completeness [%] | 96.3 | 88.6 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | 0.2M sodium formate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.50 | |
1 | 7.5 | 277 | 0.2M sodium formate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.50 |