3CG4
Crystal structure of response regulator receiver domain protein (CheY-like) from Methanospirillum hungatei JF-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-03-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 |
Unit cell lengths | 69.454, 69.454, 49.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.610 |
R-factor | 0.20213 |
Rwork | 0.201 |
R-free | 0.22596 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.312 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.630 | 1.690 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.104 | 0.950 |
Number of reflections | 17659 | |
<I/σ(I)> | 11.6 | 3 |
Completeness [%] | 99.2 | 96.3 |
Redundancy | 17.4 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 25% PEG 3350, 200mM Sodium chloride, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |