3CG3
Crystal structure of P. horikoshii periplasmic binding protein ModA/WtpA with bound tungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-02-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.21475 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.954, 59.180, 150.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.199 |
| R-free | 0.22750 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 58505 | |
| Completeness [%] | 98.8 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | Bicine, NaCl, PEG 600, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
