3CFY
Crystal structure of signal receiver domain of putative Luxo repressor protein from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 58.203, 58.203, 96.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.24763 |
| Rwork | 0.246 |
| R-free | 0.29070 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.061 | 0.820 |
| Number of reflections | 8444 | |
| <I/σ(I)> | 6.1 | 1.1 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 7.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 2.2M Ammonium sulfate pH 7.5, 100mM Sodium/potassium tartrate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
