3CFP
Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.726, 117.281, 126.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.20047 |
| Rwork | 0.197 |
| R-free | 0.26662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.104 |
| Number of reflections | 42496 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 7% PEG350 monomethyl ether, 0.1M NA CACODYLATE, 0.14M CACL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NA CACODYLATE | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 2 | NA CACODYLATE | ||
| 4 | 1 | 2 | PEG350 monomethyl ether | ||
| 5 | 1 | 2 | CaCl2 |
