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3CFH

Photoswitchable red fluorescent protein psRFP, off-state

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsMPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron siteMPG/DESY, HAMBURG
BeamlineBW6
Temperature [K]100
Detector technologyCCD
Collection date2007-11-20
DetectorMAR CCD 165 mm
Wavelength(s)1.05
Spacegroup nameC 2 2 21
Unit cell lengths97.819, 97.851, 241.827
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution12.270 - 1.750
R-factor0.20358
Rwork0.202
R-free0.23040
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3cfa
RMSD bond length0.006
RMSD bond angle1.093
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.780
High resolution limit [Å]1.7501.750
Rmerge0.0730.820
Number of reflections115191
<I/σ(I)>19.12
Completeness [%]99.499.5
Redundancy4.34
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2980.1M TRIS, 65%(w/v) MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

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