3CFD
Purple-fluorescent antibody EP2-25C10 in complex with its stilbene hapten
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.990, 72.480, 77.230 |
| Unit cell angles | 91.98, 106.11, 114.15 |
Refinement procedure
| Resolution | 29.880 - 2.500 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2NZS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.304 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 37156 | |
| <I/σ(I)> | 11.1 | 1.6 |
| Completeness [%] | 97.9 | 97.4 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | AMMONIUM SULFATE, LITHIUM SULFATE, ACETATE, PH 4.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 4.50 |
