3CFA
Anemonia sulcata red fluorescent protein asRFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.449, 98.540, 241.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.390 - 1.750 |
| R-factor | 0.20522 |
| Rwork | 0.204 |
| R-free | 0.23063 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a56 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 111019 | |
| <I/σ(I)> | 15.5 | 2 |
| Completeness [%] | 97.0 | 98.9 |
| Redundancy | 4.66 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.2M CaCl2, 28%(W/V) PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 298.0K |
