Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-03 |
| Detector | CUSTOM-MADE |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 162.660, 178.020, 321.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 4.400 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28600 |
| Structure solution method | WITH MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 4.670 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Number of reflections | 28110 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 91.0 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350, HEPES, NAF, PH 7.0, VAPOR DIFFUSION, HANGING DROP, pH 7.00, temperature 298K |
