3CEM
Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 124.383, 124.383, 123.429 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.390 - 2.470 |
| R-factor | 0.159 |
| Rwork | 0.155 |
| R-free | 0.21500 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.845 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.390 |
| High resolution limit [Å] | 2.470 |
| Number of reflections | 76587 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 7 mg/ml in 20 mM BES, 1 mM EDTA, 0.5 mM DTT, pH 6.5, 50 mM NBG, 2.5 mM AVD (AVE9423), VAPOR DIFFUSION, HANGING DROP |
