3CEG
Crystal structure of the UBC domain of baculoviral IAP repeat-containing protein 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.199, 97.815, 129.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.500 - 2.008 |
R-factor | 0.21295 |
Rwork | 0.211 |
R-free | 0.25248 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.247 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 62447 | |
<I/σ(I)> | 18.623 | 1.585 |
Completeness [%] | 93.1 | 70.1 |
Redundancy | 6.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | 298 | 11 % PEG 8000, 0.1 M Gycine buffer pH 10.0, 0.001 M DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |