3CEC

Crystal structure of a putative antidote protein of plasmid maintenance system (npun_f2943) from nostoc punctiforme pcc 73102 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97925, 0.97883
Spacegroup name	P 1 21 1
Unit cell lengths	35.070, 36.710, 50.050
Unit cell angles	90.00, 98.38, 90.00

Refinement procedure

Resolution	25.214 - 1.600
R-factor	0.168
Rwork	0.166
R-free	0.20200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.566
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.214	25.214	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.035	0.020	0.359
Number of reflections	16808	3284	3064
<I/σ(I)>	13.26	32.7	2.3
Completeness [%]	98.3	98.7	89.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 40.0% 2-Methyl-2,4-pentanediol, 5.0% PEG 8000, 0.1M Sodium cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K