3CE7
Crystal structure of toxoplasma specific mitochondrial acyl carrier protein, 59.m03510
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 64.696, 64.696, 38.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.340 - 1.640 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l0i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.052 | 0.198 |
| Number of reflections | 11276 | |
| <I/σ(I)> | 21.5 | 6.18 |
| Completeness [%] | 99.3 | 93.2 |
| Redundancy | 9.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 1.5 M NH4SO4, 0.2 M K,Na Tartrate, 0.1 M Na Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
