3CDX
Crystal structure of succinylglutamatedesuccinylase/aspartoacylase from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 152.475, 174.426, 77.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.484 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.810 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.114 | 0.519 |
| Number of reflections | 115134 | |
| <I/σ(I)> | 11.7 | 2.9 |
| Completeness [%] | 95.3 | 86.3 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 200mM Calcium acetate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, temperature 294K |
