3CDL
Crystal structure of a TetR family transcriptional regulator from Pseudomonas syringae pv. tomato str. DC3000
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915, 0.97937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.305, 53.409, 55.829 |
| Unit cell angles | 90.00, 113.25, 90.00 |
Refinement procedure
| Resolution | 33.260 - 2.360 |
| R-factor | 0.21037 |
| Rwork | 0.207 |
| R-free | 0.27514 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.260 | 2.420 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.114 | |
| Number of reflections | 14467 | |
| <I/σ(I)> | 15.47 | 1.4 |
| Completeness [%] | 96.5 | 80.7 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2M Ammonium acetate, 25% PEG 3350, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
