3CDK
Crystal structure of the co-expressed succinyl-CoA transferase A and B complex from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 69.337, 70.404, 97.996 |
Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
Resolution | 48.300 - 2.590 |
R-factor | 0.19564 |
Rwork | 0.193 |
R-free | 0.25278 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m3e |
RMSD bond length | 0.016 |
RMSD bond angle | 1.599 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 2.690 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.147 | 0.543 |
Number of reflections | 28084 | |
<I/σ(I)> | 5.9 | 2.2 |
Completeness [%] | 99.7 | 94.2 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M tri-Sodium citrate dihydrate pH 5.6, 20% Isopropanol, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |