3CDF
kI O18/O8 Y87H immunoglobulin light chain variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5241 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.959, 98.043, 73.074 |
| Unit cell angles | 90.00, 119.60, 90.00 |
Refinement procedure
| Resolution | 17.560 - 1.530 |
| R-factor | 0.19118 |
| Rwork | 0.189 |
| R-free | 0.23147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q20 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.858 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.520 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 130900 | |
| <I/σ(I)> | 14.5 | 1.9 |
| Completeness [%] | 73.7 | 13.9 |
| Redundancy | 3.31 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 295 | 1.2 M sodium citrate in 0.1 M Tris buffer, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
