3CDD
Crystal structure of prophage MuSo2, 43 kDa tail protein from Shewanella oneidensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.345, 82.445, 151.832 |
Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
Resolution | 47.690 - 2.100 |
R-factor | 0.20392 |
Rwork | 0.201 |
R-free | 0.25664 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.312 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.072 | 0.449 |
Number of reflections | 132747 | |
<I/σ(I)> | 31.76 | 2.53 |
Completeness [%] | 96.8 | 81.6 |
Redundancy | 6.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M KCl, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |