3CDC
kI O18/O8 N34I/Y87H immunoglobulin light chain variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5241 |
| Spacegroup name | P 61 |
| Unit cell lengths | 73.832, 73.829, 97.541 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.520 - 1.530 |
| R-factor | 0.19118 |
| Rwork | 0.189 |
| R-free | 0.23147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q20 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.858 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.520 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 64183 | |
| <I/σ(I)> | 12 | 1.4 |
| Completeness [%] | 93.8 | 45.1 |
| Redundancy | 6.4 | 1.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | 1.1 M sodium citrate in 0.1 M Tris buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
