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3CD1

Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 5.0.1
Synchrotron siteALS
Beamline5.0.1
Temperature [K]100
Detector technologyCCD
Collection date2007-08-11
DetectorADSC QUANTUM 210
Wavelength(s)1.00
Spacegroup nameP 21 21 21
Unit cell lengths51.187, 62.363, 68.803
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 1.312
R-factor0.16464
Rwork0.164
R-free0.18377
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.404
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.360
High resolution limit [Å]1.3121.312
Number of reflections52564
Completeness [%]98.692.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82980.1M tris, 24% PEG 1550, 0.04M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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