3CCS
Structure of Anisomycin resistant 50S Ribosomal Subunit: 23S rRNA mutation G2482A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-28 |
| Detector | ADSC |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.241, 299.185, 575.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| Rwork | 0.179 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
| Rmerge | 0.091 | 0.044 | 0.711 |
| Number of reflections | 371978 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 97.7 | 97.7 | 96.9 |
| Redundancy | 3.5 | 4.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, sitting drop, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | NH4CL | ||
| 4 | 1 | 1 | MGCl2 | ||
| 5 | 1 | 2 | PEG6000 | ||
| 6 | 1 | 2 | KCL | ||
| 7 | 1 | 2 | NH4CL | ||
| 8 | 1 | 2 | MGCl2 |
