3CCS
Structure of Anisomycin resistant 50S Ribosomal Subunit: 23S rRNA mutation G2482A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-28 |
Detector | ADSC |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 212.241, 299.185, 575.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.950 |
Rwork | 0.179 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
Rmerge | 0.091 | 0.044 | 0.711 |
Number of reflections | 371978 | ||
<I/σ(I)> | 11.6 | ||
Completeness [%] | 97.7 | 97.7 | 96.9 |
Redundancy | 3.5 | 4.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, sitting drop, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | NH4CL | ||
4 | 1 | 1 | MGCl2 | ||
5 | 1 | 2 | PEG6000 | ||
6 | 1 | 2 | KCL | ||
7 | 1 | 2 | NH4CL | ||
8 | 1 | 2 | MGCl2 |