3CCP
X-RAY STRUCTURES OF RECOMBINANT YEAST CYTOCHROME C PEROXIDASE AND THREE HEME-CLEFT MUTANTS PREPARED BY SITE-DIRECTED MUTAGENESIS
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.570, 74.380, 45.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.200 |
| R-factor | 0.165 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.037 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.036 * |
| Number of reflections | 17679 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | MPD | 20 (%(v/v)) | |
| 4 | 1 | reservoir | MPD | 30 (%(v/v)) |
