3CCK
Human CD69
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 |
| Unit cell lengths | 85.693, 85.693, 61.881 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.050 - 1.800 |
| R-factor | 0.19239 |
| Rwork | 0.191 |
| R-free | 0.22285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8i |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.540 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.575 |
| Number of reflections | 22276 | |
| <I/σ(I)> | 29.5 | 2.6 |
| Completeness [%] | 98.0 | 76.5 |
| Redundancy | 7.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% PEG 3400, 0.1M Arginine.HCl, 10mM CaCl2, 1mM NaN3, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
