3CC7
Structure of Anisomycin resistant 50S Ribosomal Subunit: 23S rRNA mutation C2487U
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-18 |
| Detector | ADSC |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.832, 299.896, 576.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.830 - 2.700 |
| Rwork | 0.184 |
| R-free | 0.22600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.125 | 0.877 |
| Number of reflections | 509913 | |
| <I/σ(I)> | 12.25 | 1.8 |
| Completeness [%] | 98.2 | 98.7 |
| Redundancy | 5.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.8, sitting drop, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | NH4CL | ||
| 4 | 1 | 1 | MGCl2 | ||
| 5 | 1 | 2 | PEG6000 | ||
| 6 | 1 | 2 | KCL | ||
| 7 | 1 | 2 | NH4CL | ||
| 8 | 1 | 2 | MGCl2 |
