3CC4
Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.780, 299.084, 573.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.850 - 2.700 |
Rwork | 0.202 |
R-free | 0.24400 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.770 |
High resolution limit [Å] | 2.700 | 6.500 | 2.700 |
Rmerge | 0.106 | 0.035 | |
Number of reflections | 492663 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 99.9 | 99.8 | 99.1 |
Redundancy | 6.8 | 6.8 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.8, sitting drop, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | NH4CL | ||
4 | 1 | 1 | MGCl2 | ||
5 | 1 | 2 | PEG6000 | ||
6 | 1 | 2 | KCL | ||
7 | 1 | 2 | NH4CL | ||
8 | 1 | 2 | MGCl2 |