3CBW
Crystal structure of the YdhT protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.728, 69.619, 72.505 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.269 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.17200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.548 | 1.330 |
| High resolution limit [Å] | 1.269 | 1.269 |
| Rmerge | 0.095 | 0.439 |
| Number of reflections | 145644 | |
| <I/σ(I)> | 15.1 | 3.3 |
| Completeness [%] | 95.5 | 88.5 |
| Redundancy | 7.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.5 | 294 | 100mM Citric acid pH 3.5, 25% PEG 3350, VAPOR DIFFUSION, temperature 294K |
