3CBF
Crystal structure of LysN, alpha-aminoadipate aminotransferase, from Thermus thermophilus HB27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.279, 93.243, 150.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.690 - 1.670 |
| R-factor | 0.20415 |
| Rwork | 0.202 |
| R-free | 0.24709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z1y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.066 | 0.556 |
| Number of reflections | 91233 | |
| <I/σ(I)> | 30.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 18% PEG3350, 0.2M potassium fluoride, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
