3CB6
Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9699 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.980, 193.670, 89.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 1.840 |
R-factor | 0.19371 |
Rwork | 0.192 |
R-free | 0.22472 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cb5 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.258 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 1.950 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 47711 | |
<I/σ(I)> | 20.49 | 4.1 |
Completeness [%] | 99.6 | 98.4 |
Redundancy | 7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% PEG 300, 0.1M Mes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |