3CB5
Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.070, 101.650, 103.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.120 - 2.050 |
R-factor | 0.20767 |
Rwork | 0.206 |
R-free | 0.24466 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.419 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.550 | 2.170 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 64731 | |
<I/σ(I)> | 11 | 2.19 |
Completeness [%] | 99.6 | 99 |
Redundancy | 3.65 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 13% PEG 2000, 0.1M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |