3CAZ

Crystal structure of a BAR protein from Galdieria sulphuraria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.96411
Spacegroup name	P 61 2 2
Unit cell lengths	78.962, 78.962, 456.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.168 - 3.344
R-factor	0.217
Rwork	0.215
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.168	47.168	3.470
High resolution limit [Å]	3.344	7.220	3.344
Rmerge	0.085	0.046	0.858
Number of reflections	13119
<I/σ(I)>	11.425		1.622
Completeness [%]	98.3	94.1	98.2
Redundancy	12.8	11.8	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Protein solution (10 mg/mL Se-Met protein, 0.05 M Sodium chloride, 0.0003 M TCEP, 0.005 M Bis-Tris pH 7.0) mixed in a 1:1 ratio with the Well solution (27% MPD, 0.12 M Magnesium chloride, 0.1 M MES/Acetate pH 5.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K