3CAN
Crystal structure of a domain of pyruvate-formate lyase-activating enzyme from Bacteroides vulgatus ATCC 8482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.768, 73.768, 101.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.800 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.18200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.144 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.076 | 0.700 |
Number of reflections | 26654 | |
<I/σ(I)> | 47 | 4.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.2 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 60% v/v Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |