3CAM
Crystal structure of the cold shock domain protein from Neisseria meningitidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 59.369, 59.369, 89.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.23646 |
Rwork | 0.233 |
R-free | 0.29663 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.022 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.127 | 0.834 |
Number of reflections | 5271 | |
<I/σ(I)> | 29.2 | 1.7 |
Completeness [%] | 98.5 | 88.8 |
Redundancy | 23.9 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 0.8 M Sodium dihydrogen phosphate, 1.2 M di-potassium hydrogen phosphate, 100 mM Acetate pH 4.5, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |