3CAF

Crystal Structure of hFGFR2 D2 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298
Detector technology	CCD
Collection date	2006-10-27
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	42.500, 79.900, 37.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.760 - 1.960
R-factor	0.191
Rwork	0.187
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.032
RMSD bond angle	2.082
Data reduction software	CrystalClear
Data scaling software	d*TREK (9.2SSI)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	16.760	16.760	2.030
High resolution limit [Å]	1.960	4.200	1.960
Rmerge	0.048	0.021	0.386
Total number of observations		3587	1747
Number of reflections	9401
<I/σ(I)>	14.4	51.7	1.9
Completeness [%]	98.1	99.7	84.9
Redundancy	3.39	3.39	2.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20%(w/v) PEG3350, 0.1M (NH3)2SO4, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K