3CAE
Structure of NNQQNY as an insert in T7 endonuclease I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.408, 140.343, 88.508 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 90.000 - 3.000 |
| Rwork | 0.240 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fzr residues 18-43 of molecule A and residues 51-145 of molecule B |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.349 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 43123 | |
| <I/σ(I)> | 14.2 | 1.6 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.6 M ammonium sulfate, 0.1 M sodium citrate, 2% dimethyl formamide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
