3CAD
Crystal structure of Natural Killer Cell Receptor, Ly49G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.378, 56.196, 33.761 |
| Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.22971 |
| Rwork | 0.227 |
| R-free | 0.28636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.777 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.100 |
| Number of reflections | 8105 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 81.5 | 45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% (weight/volume) PEG 4000, 0.1 mM Tris-HCl (pH 8.5), and 0.2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
