3C9Q
Crystal structure of the uncharacterized human protein C8orf32 with bound peptide
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 34.322, 64.039, 113.660 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 分解能 | 32.857 - 1.500 |
| R因子 | 0.162 |
| Rwork | 0.161 |
| R-free | 0.18000 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.545 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 32.857 | 32.857 | 1.530 |
| 分解能 [Å] (高) | 1.498 | 3.700 | 1.498 |
| Rmerge_l_obs | 0.105 | 0.058 | 0.543 |
| 独立反射数 | 40943 | ||
| <I/σ(I)> | 12.075 | 2.526 | |
| 完全性 [%] | 99.5 | 99.5 | 95.9 |
| 冗長性 | 12.2 | 12.6 | 6.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 291 | Protein solution (10 mg/mL Se-Met protein, 0.050 M Sodium chloride, 0.003 M Sodium azide, 0.0003 M TCEP, Bis-Tris pH 7.0) mixed in a 1:1 ratio with the Well solution (1% Ethylene glycol, 1.8 M Ammonium sulfate, 0.10 M MES pH 6.0). Cryoprotected in four stages with well solution using 0 to 25 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
