3C9Q

Crystal structure of the uncharacterized human protein C8orf32 with bound peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-06
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	34.322, 64.039, 113.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.857 - 1.500
R-factor	0.162
Rwork	0.161
R-free	0.18000
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.545
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.857	32.857	1.530
High resolution limit [Å]	1.498	3.700	1.498
Rmerge	0.105	0.058	0.543
Number of reflections	40943
<I/σ(I)>	12.075		2.526
Completeness [%]	99.5	99.5	95.9
Redundancy	12.2	12.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			291	Protein solution (10 mg/mL Se-Met protein, 0.050 M Sodium chloride, 0.003 M Sodium azide, 0.0003 M TCEP, Bis-Tris pH 7.0) mixed in a 1:1 ratio with the Well solution (1% Ethylene glycol, 1.8 M Ammonium sulfate, 0.10 M MES pH 6.0). Cryoprotected in four stages with well solution using 0 to 25 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K