3C9D
Crystal structure of Vps75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.805, 86.085, 85.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.844 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.408 |
| Number of reflections | 37640 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 4.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20 mM Tris, 100 mM KCl, 10 mM MgCl(2), 35% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
