3C99
Structural Basis of Histone H4 Recognition by p55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-05-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 151.121, 151.121, 98.013 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.219 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | initial model from Se Data |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 28772 | |
| <I/σ(I)> | 44.9 | 2.4 |
| Completeness [%] | 99.7 | 97.2 |
| Redundancy | 14.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
