3C90
The 1.25 A Resolution Structure of Phosphoribosyl-ATP Pyrophosphohydrolase from Mycobacterium tuberculosis, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.702, 43.119, 67.547 |
| Unit cell angles | 90.00, 96.43, 90.00 |
Refinement procedure
| Resolution | 33.560 - 1.790 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y6x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 31912 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1M Sodium formate, 0.1M Sodium iodide, 0.1 mM Calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
