3C8U

Crystal structure of putative fructose transport system kinase (YP_612366.1) from Silicibacter sp. TM1040 at 1.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97978, 0.91837, 0.97964
Spacegroup name	H 3 2
Unit cell lengths	102.280, 102.280, 116.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.267 - 1.950
R-factor	0.167
Rwork	0.165
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.267	35.267	2.020
High resolution limit [Å]	1.950	4.190	1.950
Rmerge	0.055	0.038	0.263
Number of reflections	15859	1822	1715
<I/σ(I)>	19.95	31.6	7.2
Completeness [%]	91.6	99.3	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	NANODROP, 23.1% PEG 3350, 0.214M Sodium fluoride, VAPOR DIFFUSION, SITTING DROP, temperature 277K