3C8O
The Crystal Structure of RraA from PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 98.679, 98.679, 180.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.22222 |
| Rwork | 0.221 |
| R-free | 0.24898 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.986 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.245 |
| Number of reflections | 39040 | |
| <I/σ(I)> | 11.2 | 8 |
| Completeness [%] | 95.0 | 94.9 |
| Redundancy | 8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100mM Tris-HCl pH 8.5, 30% (v/v) PEG 200, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
