3C8M
Crystal structure of homoserine dehydrogenase from Thermoplasma volcanium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.404, 108.404, 60.458 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.200 - 1.900 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.17700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.102 | 0.763 |
| Number of reflections | 32528 | |
| <I/σ(I)> | 8 | 3.47 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M Lithium sulfate, 0.12 M Tris buffer, 2 M Ammonium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
