3C8I
Crystal structure of a putative membrane protein from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.374, 99.837, 124.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.260 - 1.950 |
| R-factor | 0.20185 |
| Rwork | 0.200 |
| R-free | 0.23164 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.430 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.260 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.099 | 0.736 |
| Number of reflections | 22035 | |
| <I/σ(I)> | 40.13 | 2.31 |
| Completeness [%] | 99.5 | 99.94 |
| Redundancy | 9.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05M Calcium chloride dihydrate, 0.1M Bis-Tris, 30% v/v PEG MME 550, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
